Journal: Journal of Materials Chemistry. B
Article Title: Barcoding chemical modifications into nucleic acids improves drug stability in vivo
doi: 10.1039/c8tb01642a
Figure Lengend Snippet: Barcodes with 5 phosphorothioates and 5 2- O -methyl modifications exhibit increased stability in cells and in mice. (A) Enrichment of each DNA barcode compared to barcodes with no modifications in vitro in HEK293T cells at 24 hours. (B) Enrichment of each DNA barcode compared to barcodes with no modifications in vivo from lung endothelial cells at 24 hours. Correlation of in vivo enrichment compared to (C) 1 hour, (D) 3 hours, (E) 24 hours incubation in 10% mouse serum, and (F) in vitro in HEK 293T cells.
Article Snippet: In vitro experiments were performed using HEK293T cells cultured in DMEM/F-12 50/50 media (Corning) supplemented by 10% (v/v) FBS (VWR) and 1% (v/v) penicillin–streptomycin (VWR).
Techniques: In Vitro, In Vivo, Incubation